TY - JOUR
T1 - Leaf-Encapsulated Vaccines
T2 - Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves
AU - Liu, Pei Feng
AU - Wang, Yanhan
AU - Ulrich, Robert G.
AU - Simmons, Christopher W.
AU - Vandergheynst, Jean S.
AU - Gallo, Richard L.
AU - Huang, Chun Ming
N1 - Publisher Copyright:
© 2018 Pei-Feng Liu et al.
PY - 2018
Y1 - 2018
N2 - Transgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression. Here, we introduce a new vaccine modality in which Staphylococcal enterotoxin B (SEB) genes are agroinfiltrated into radishes (Raphanw sativus L.), resulting in transient expression and accumulation of SEB in planta. This approach can simultaneously express multiple antigens in a single leaf. Furthermore, the potential of high-throughput vaccine production was demonstrated by simultaneously agroinfiltrating multiple radish leaves using a multichannel pipette. The expression of SEB was detectable in two leaf cell types (epidermal and guard cells) in agroinfiltrated leaves. ICR mice intranasally immunized with homogenized leaves agroinfiltrated with SEB elicited detectable antibody to SEB and displayed protection against SEB-induced interferon-gamma (IFN-γ) production. The concept of encapsulating antigens in leaves rather than purifying them for immunization may facilitate rapid vaccine production during an epidemic disease.
AB - Transgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression. Here, we introduce a new vaccine modality in which Staphylococcal enterotoxin B (SEB) genes are agroinfiltrated into radishes (Raphanw sativus L.), resulting in transient expression and accumulation of SEB in planta. This approach can simultaneously express multiple antigens in a single leaf. Furthermore, the potential of high-throughput vaccine production was demonstrated by simultaneously agroinfiltrating multiple radish leaves using a multichannel pipette. The expression of SEB was detectable in two leaf cell types (epidermal and guard cells) in agroinfiltrated leaves. ICR mice intranasally immunized with homogenized leaves agroinfiltrated with SEB elicited detectable antibody to SEB and displayed protection against SEB-induced interferon-gamma (IFN-γ) production. The concept of encapsulating antigens in leaves rather than purifying them for immunization may facilitate rapid vaccine production during an epidemic disease.
UR - http://www.scopus.com/inward/record.url?scp=85042552218&partnerID=8YFLogxK
U2 - 10.1155/2018/3710961
DO - 10.1155/2018/3710961
M3 - 期刊論文
C2 - 29577048
AN - SCOPUS:85042552218
VL - 2018
JO - Journal of Immunology Research
JF - Journal of Immunology Research
SN - 2314-8861
M1 - 3710961
ER -