Investigation of the binding affinity of C-terminal domain of SARS coronavirus nucleocapsid protein to nucleotide using AlGaN/GaN high electron mobility transistors

You Ren Hsu, Geng Yen Lee, Jen Inn Chyi, Chung Ke Chang, Chih Cheng Huang, Chen Pin Hsu, Tai Huang Huang, Fan Ren, Yu Lin Wang

研究成果: 書貢獻/報告類型會議論文篇章同行評審

2 引文 斯高帕斯(Scopus)

摘要

In this article, we used AlGaN/GaN high electron mobility transistors (HEMTs) to construct the first label-free semiconductor-sensor-based binding assay to our knowledge. Our results suggested that the nucleotide-c terminal domain of SARS coronavirus (SARS-CoV) nucleocapsid protein interaction is a two-step binding event with two dissociation constants (Kd1 = 0.052 nM, and Kd2 = 51.24nM) extracted by using the modified two-binding-site Langmuir isotherm equation proposed here. We found that there were at least two protein binding sites on the specific 41-base SARS-CoV double-stranded DNA (dsDNA) genome (29,580-29621) conjugated with a 20-mer poly-dT tail. This result presented a high binding affinity is comparable with the antibody-antigen reaction, and suggested this designed dsDNA could be treated as an aptamer for SARS-CoV N protein capture.

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主出版物標題IEEE SENSORS 2012 - Proceedings
DOIs
出版狀態已出版 - 2012
事件11th IEEE SENSORS 2012 Conference - Taipei, Taiwan
持續時間: 28 10月 201231 10月 2012

出版系列

名字Proceedings of IEEE Sensors

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???event.eventtypes.event.conference???11th IEEE SENSORS 2012 Conference
國家/地區Taiwan
城市Taipei
期間28/10/1231/10/12

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