@article{8a658d04436e40068cabb777ad82804f,
title = "Investigation of C-terminal domain of SARS nucleocapsid protein-Duplex DNA interaction using transistors and binding-site models",
abstract = "AlGaN/GaN high electron mobility transistors (HEMTs) were used to sense the binding between double stranded DNA (dsDNA) and the severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein (N protein). The sensing signals were the drain current change of the HEMTs induced by the protein-dsDNA binding. Binding-site models using surface coverage ratios were utilized to analyze the signals from the HEMT-based sensors to extract the dissociation constants and predict the number of binding sites. Two dissociation constants, KD1 = 0.0955 nM, KD2 = 51.23 nM, were obtained by fitting the experimental results into the two-binding-site model. The result shows that this technique is more competitive than isotope-labeling electrophoretic mobility shift assay (EMSA). We demonstrated that AlGaN/GaN HEMTs were highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleotide-protein interaction.",
keywords = "Binding sites, Dissociation constants, GaN, HEMTs, SARS, Sensors",
author = "Hsu, {You Ren} and Kang, {Yen Wen} and Fang, {Jung Ying} and Lee, {Geng Yen} and Chyi, {Jen Inn} and Chang, {Chung Ke} and Huang, {Chih Cheng} and Hsu, {Chen Pin} and Huang, {Tai Huang} and Huang, {Yu Fen} and Sun, {Yuh Chang} and Hsu, {Chia Hsien} and Chen, {Chih Chen} and Li, {Sheng Shian} and Yeh, {J. Andrew} and Yao, {Da Jeng} and Fan Ren and Wang, {Yu Lin}",
note = "Funding Information: This work was partially supported by National Science Council grant (No. 99B20495A ) and by the research grant ( 100N2049E1 ) at National Tsing Hua University . We thank Dr. Jen-Inn Chyi, and Dr. Chung-ke Chang and Dr. Tai-huang Huang for providing us the AlGaN/GaN epi wafers and the SARS-N protein, respectively, and for consulting as well. ",
year = "2014",
month = mar,
day = "31",
doi = "10.1016/j.snb.2013.11.087",
language = "???core.languages.en_GB???",
volume = "193",
pages = "334--339",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
}