Interfacing multiplexed microfluidics to MALDI-MS by high-throughput robotic spotting

C. W. Tsao, T. Song, C. F. Chen, J. Liu, C. C. Chang, D. L. Devoe

研究成果: 書貢獻/報告類型會議論文篇章同行評審

摘要

Direct and automated spotting of analyte from multichannel microfluidic chips is demonstrated as a simple, robust, and high-throughput method for interfacing arrays of parallel microchannels with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Using cyclic olefin copolymer (COC) chips containing 8 parallel 100 µm x 46 µm channels connected to a single input port, excellent spotting volume repeatability and MALDI-MS signal uniformity are achieved for a panel of sample peptides. Using the robotic spotting approach, chip-based reversed-phase liquid chromatography (RPLC) separations are interfaced with nanofilament silicon LDI-MS, demonstrating the potential for this approach towards high-throughput on-chip bioanalysis coupled seamlessly with back-end mass spectrometry.

原文???core.languages.en_GB???
主出版物標題2008 Solid-State Sensors, Actuators, and Microsystems Workshop
編輯Kimberly L. Turner, Leland Spangler
發行者Transducer Research Foundation
頁面110-113
頁數4
ISBN(電子)0964002477, 9780964002470
DOIs
出版狀態已出版 - 2008
事件2008 Solid-State Sensors, Actuators, and Microsystems Workshop - Hilton Head Island, United States
持續時間: 1 6月 20085 6月 2008

出版系列

名字Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop

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???event.eventtypes.event.conference???2008 Solid-State Sensors, Actuators, and Microsystems Workshop
國家/地區United States
城市Hilton Head Island
期間1/06/085/06/08

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