FORMOSAT-5 satellite was launched into a sun-synchronous orbit at 720 km altitude with 98.28° inclination on 25 August 2017. The onboard scientific payload, Advanced Ionospheric Probe (AIP) is capable of measuring topside ionospheric ion density, cross-track flow velocities, ion composition and temperature, and electron temperature. Initial observations of nighttime midlatitude ionospheric density and vertical flow velocity variations at 2230 LT sector during a few quiet magnetic days in December 2017 are studied here. Longitudinal density variations in the equatorward edge of midlatitude ionospheric trough (MIT) region are noticed. Accompanied with this density variation, the vertical flow velocities also behave differently. Although the density difference has been stated due to zonal wind effect related to the declination of the geomagnetic field lines, the vertical flow velocity variation seems to play the opposite role. All these density and vertical flow observations in the northern winter hemisphere can only be explained by the longitudinal differences in the diffusion velocity coming down from the protonsphere (plasmasphere). In addition, the hemispheric asymmetry in the vertical flow velocity can also be explained by the interaction between the topside ionosphere and the protonsphere. The observed vertical flow variations near MIT at different longitudes should present a new potential tool for the study of MIT formation.