In vivo developmental biology study using noninvasive multi-harmonic generation microscopy

Shi Wei Chu, Szu Yu Chen, Tsung Han Tsai, Tzu Ming Liu, Cheng Yung Lin, Huai Jen Tsai, Chi Kuang Sun

研究成果: 雜誌貢獻期刊論文同行評審

157 引文 斯高帕斯(Scopus)

摘要

Morphological changes and complex developmental processes inside vertebrate embryos are difficult to observe noninvasively with millimeter-penetration and sub-micrometer-resolution at the same time. By using higher harmonic generation, including second and third harmonics, as the microscopic contrast mechanism, optical noninvasiveness can be achieved due to the virtual-level-transition characteristic. The intrinsic nonlinearity of harmonic generations provides optical sectioning capability while the selected 1230-nm near-infrared light source provides the deep-penetration ability. The complicated development within a ∼1.5-mm thick zebrafish (Danio rerio) embryo from initial cell proliferation, gastrulation, to tissue formation can all be observed clearly in vivo without any treatment on the live specimen.

原文???core.languages.en_GB???
頁(從 - 到)3093-3099
頁數7
期刊Optics Express
11
發行號23
DOIs
出版狀態已出版 - 11月 2003

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