Improving the remaining activity of lignocellulolytic enzymes by membrane entrapment

Ken Lin Chang, Jitladda Thitikorn-amorn, Shan He Chen, Jung Feng Hsieh, Khanok Ratanakhanokchai, Po Jung Huang, Ting Chia Lin, Shui Tein Chen

研究成果: 雜誌貢獻期刊論文同行評審

16 引文 斯高帕斯(Scopus)


The production of bioethanol by the conversion of lignocellulosic waste has attracted much interest in recent years because of its low cost and great potential availability. However, the high cost of the enzyme required for this conversion is often considered to be the major bottleneck in the commercial lignocellulosic ethanol industry. In this work, the hydrolysis of rice straw by free and entrapped lignocellulolytic enzymes (cellulase, xylanase and laccase) was carried out at pH 5.5 and 37 °C. The hydrolysis of rice straw by enzymes entrapped in a membrane produced a higher monosaccharide content: 601.05. mg/g rice straw for entrapped enzymes vs. 465.46. mg/g rice straw for free enzymes. This study has shown that enzyme entrapment is an important technique for the efficient use and reuse of enzymes in industrial applications and also for the rapid separation of saccharide products from the reaction medium, thus improving the remaining enzymatic activities.

頁(從 - 到)519-523
期刊Bioresource Technology
出版狀態已出版 - 1月 2011


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