Improving resolution of second harmonic generation microscopy via scanning structured illumination

Chia Hua Yeh, Cheng Zn Tan, Ching Hsiao Arthur Cheng, Jui Ting Hung, Szu Yu Chen

研究成果: 雜誌貢獻期刊論文同行評審

7 引文 斯高帕斯(Scopus)

摘要

Second harmonic generation microscopy (SHGM) is a well-known technique for examining the noncentrosymmetric structures in biomedical research. However, without real-state transitions, fluorescence-based superresolution methods cannot be applied. To improve the resolution, fringe-scanning SHGM (FS-SHGM), which combines SHGM with structured illumination based on point-scanning, is introduced in this paper. The scanning path was modulated to generate illumination patterns. For the coherent parts of SHG signals, a mathematical model of image formation and reconstruction was established. Both simulations and experiments showed a resolution improvement factor of ~1.4 in the lateral and 1.56 in the axial directions for chicken tendons and mouse skin.

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文章編號#342072
頁(從 - 到)6081-6090
頁數10
期刊Biomedical Optics Express
9
發行號12
DOIs
出版狀態已出版 - 1 12月 2018

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