Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de Novo Approach: Size-Selective Sheltering of Catalase in Metal-Organic Framework Microcrystals

Fa Kuen Shieh, Shao Chun Wang, Chia I. Yen, Chang Cheng Wu, Saikat Dutta, Lien Yang Chou, Joseph V. Morabito, Pan Hu, Ming Hua Hsu, Kevin C.W. Wu, Chia Kuang Tsung

研究成果: 雜誌貢獻期刊論文同行評審

541 引文 斯高帕斯(Scopus)

摘要

We develop a new concept to impart new functions to biocatalysts by combining enzymes and metal-organic frameworks (MOFs). The proof-of-concept design is demonstrated by embedding catalase molecules into uniformly sized ZIF-90 crystals via a de novo approach. We have carried out electron microscopy, X-ray diffraction, nitrogen sorption, electrophoresis, thermogravimetric analysis, and confocal microscopy to confirm that the ∼10 nm catalase molecules are embedded in 2 μm single-crystalline ZIF-90 crystals with ∼5 wt % loading. Because catalase is immobilized and sheltered by the ZIF-90 crystals, the composites show activity in hydrogen peroxide degradation even in the presence of protease proteinase K.

原文???core.languages.en_GB???
頁(從 - 到)4276-4279
頁數4
期刊Journal of the American Chemical Society
137
發行號13
DOIs
出版狀態已出版 - 8 4月 2015

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