Humic acid induced genotoxicity in human peripheral blood lymphocytes using comet and sister chromatid exchange assay

You Cheng Hseu, Ssu Ching Chen, Ya Lai Chen, Jing Yi Chen, Mei Ling Lee, Fung Jou Lu, Fang Yang Wu, Jim Shoung Lai, Hsin Ling Yang

研究成果: 雜誌貢獻期刊論文同行評審

24 引文 斯高帕斯(Scopus)

摘要

Humic acid (HA) in well water used by the inhabitants for drinking is one of the possible etiological factors for blackfoot disease (BFD). Moreover, within BFD endemic areas cancers occur at significantly higher rates than in areas free of BFD. In this study, the genotoxic potential of HA is assessed using human peripheral blood lymphocytes. The cells were exposed to HA (0-200 μg/mL for 2 h), and the induction of DNA primary damage in cellular DNA was evaluated by single-cell gel electrophoresis (comet assay). HA-induced DNA damage was decreased by superoxide (O2-), hydrogen peroxide (H2O2), and reactive oxygen species (ROS) scavengers (superoxide dismutase, catalase, and Trolox), and nitric oxide (NO) synthase inhibitors (NG-nitro-l-arginine methyl ester and NG-methyl-l-arginine). Moreover, formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease III (Endo III), known to catalyze the excision of oxidized bases, increase the amount of DNA migration in HA-treated cells. Pretreatment of the cells with both the Ca2+-chelator BAPTA and EGTA completely inhibited HA-induced DNA damage, indicating that HA-induced changes in Ca2+-homeostasis are the predominant pathways for the HA induction of genotoxicity. Furthermore, sister chromatid exchange was found in the HA-treated lymphocytes. Our findings suggest that HA can induce oxidative DNA damage and genotoxicity in human lymphocytes.

原文???core.languages.en_GB???
頁(從 - 到)784-791
頁數8
期刊Journal of Hazardous Materials
153
發行號1-2
DOIs
出版狀態已出版 - 1 5月 2008

指紋

深入研究「Humic acid induced genotoxicity in human peripheral blood lymphocytes using comet and sister chromatid exchange assay」主題。共同形成了獨特的指紋。

引用此