Generation of pluripotent stem cells without the use of genetic material

Akon Higuchi, Qing Dong Ling, S. Suresh Kumar, Murugan A. Munusamy, Abdullah A. Alarfaj, Yung Chang, Shih Hsuan Kao, Ke Chen Lin, Han Chow Wang, Akihiro Umezawa

研究成果: 雜誌貢獻回顧評介論文同行評審

50 引文 斯高帕斯(Scopus)

摘要

Induced pluripotent stem cells (iPSCs) provide a platform to obtain patient-specific cells for use as a cell source in regenerative medicine. Although iPSCs do not have the ethical concerns of embryonic stem cells, iPSCs have not been widely used in clinical applications, as they are generated by gene transduction. Recently, iPSCs have been generated without the use of genetic material. For example, protein-induced PSCs and chemically induced PSCs have been generated by the use of small and large (protein) molecules. Several epigenetic characteristics are important for cell differentiation; therefore, several small-molecule inhibitors of epigenetic-modifying enzymes, such as DNA methyltransferases, histone deacetylases, histone methyltransferases, and histone demethylases, are potential candidates for the reprogramming of somatic cells into iPSCs. In this review, we discuss what types of small chemical or large (protein) molecules could be used to replace the viral transduction of genes and/or genetic reprogramming to obtain human iPSCs.

原文???core.languages.en_GB???
頁(從 - 到)26-42
頁數17
期刊Laboratory Investigation
95
發行號1
DOIs
出版狀態已出版 - 31 1月 2015

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