Fluid flow shear stress stimulation on a multiplex microfluidic device for rat bone marrow stromal cell differentiation enhancement

Chia Wen Tsao, Yu Che Cheng, Jhih Hao Cheng

研究成果: 雜誌貢獻期刊論文同行評審

10 引文 斯高帕斯(Scopus)

摘要

Microfluidic devices provide low sample consumption, high throughput, high integration, and good environment controllability advantages. An alternative to conventional bioreactors, microfluidic devices are a simple and effective platform for stem cell investigations. In this study, we describe the design of a microfluidic device as a chemical and mechanical shear stress bioreactor to stimulate rat bone marrow stromal cells (rBMSCs) into neuronal cells. 1-methyl-3-isobutylxanthine (IBMX) was used as a chemical reagent to induce rBMSCs differentiation into neurons. Furthermore, the shear stress applied to rBMSCs was generated by laminar microflow in the microchannel. Four parallel microfluidic chambers were designed to provide a multiplex culture platform, and both the microfluidic chamber-to-chamber, as well as microfluidic device-to-device, culture stability were evaluated. Our research shows that rBMSCs were uniformly cultured in the microfluidic device and differentiated into neuronal cells with IBMX induction. A three-fold increase in the neuronal cell differentiation ratio was noted when rBMSCs were subjected to both IBMX and fluid flow shear stress stimulation. Here, we propose a microfluidic device which is capable of providing chemical and physical stimulation, and could accelerate neuronal cell differentiation from bone marrow stromal cells.

原文???core.languages.en_GB???
頁(從 - 到)1996-2009
頁數14
期刊Micromachines
6
發行號12
DOIs
出版狀態已出版 - 2015

指紋

深入研究「Fluid flow shear stress stimulation on a multiplex microfluidic device for rat bone marrow stromal cell differentiation enhancement」主題。共同形成了獨特的指紋。

引用此