TY - JOUR
T1 - Fabrication of nano-mosquitocides using chitosan from crab shells
T2 - Impact on non-target organisms in the aquatic environment
AU - Murugan, Kadarkarai
AU - Anitha, Jaganathan
AU - Dinesh, Devakumar
AU - Suresh, Udaiyan
AU - Rajaganesh, Rajapandian
AU - Chandramohan, Balamurugan
AU - Subramaniam, Jayapal
AU - Paulpandi, Manickam
AU - Vadivalagan, Chitravel
AU - Amuthavalli, Pandiyan
AU - Wang, Lan
AU - Hwang, Jiang Shiou
AU - Wei, Hui
AU - Alsalhi, Mohamad Saleh
AU - Devanesan, Sandhanasamy
AU - Kumar, Suresh
AU - Pugazhendy, Kannaiyan
AU - Higuchi, Akon
AU - Nicoletti, Marcello
AU - Benelli, Giovanni
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Mosquitoes are arthropods of huge medical and veterinary relevance, since they vector pathogens and parasites of public health importance, including malaria, dengue and Zika virus. Currently, nanotechnology is considered a potential eco-friendly approach in mosquito control research. We proposed a novel method of biofabrication of silver nanoparticles (AgNP) using chitosan (Ch) from crab shells. Ch-AgNP nanocomposite was characterized by UV–vis spectroscopy, FTIR, SEM, EDX and XRD. Ch-AgNP were tested against larvae and pupae of the malaria vector Anopheles stephensi obtaining LC50 ranging from 3.18 ppm (I) to 6.54 ppm (pupae). The antibacterial properties of Ch-AgNP were proved against Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi, while no growth inhibition was reported in assays conducted on Proteus vulgaris. Concerning non-target effects, in standard laboratory considtions the predation efficiency of Danio rerio zebrafishes was 68.8% and 61.6% against I and II instar larvae of A. stephensi, respectively. In a Ch-AgNP-contaminated environment, fish predation was boosted to 89.5% and 77.3%, respectively. Quantitative analysis of antioxidant enzymes SOD, CAT and LPO from hepatopancreas of fresh water crabs Paratelphusa hydrodromous exposed for 16 days to a Ch-AgNP-contaminated aquatic environment were conducted. Notably, deleterious effects of Ch-AgNP contaminating aquatic enviroment on the non-target crab P. hydrodromous were observed, particularly when doses higher than 8–10 ppm are tested. Overall, this research highlights the potential of Ch-AGNP for the development of newer control tools against young instar populations of malaria mosquitoes, also highlighting some risks concerned the employ of nanoparticles in aquatic environments.
AB - Mosquitoes are arthropods of huge medical and veterinary relevance, since they vector pathogens and parasites of public health importance, including malaria, dengue and Zika virus. Currently, nanotechnology is considered a potential eco-friendly approach in mosquito control research. We proposed a novel method of biofabrication of silver nanoparticles (AgNP) using chitosan (Ch) from crab shells. Ch-AgNP nanocomposite was characterized by UV–vis spectroscopy, FTIR, SEM, EDX and XRD. Ch-AgNP were tested against larvae and pupae of the malaria vector Anopheles stephensi obtaining LC50 ranging from 3.18 ppm (I) to 6.54 ppm (pupae). The antibacterial properties of Ch-AgNP were proved against Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi, while no growth inhibition was reported in assays conducted on Proteus vulgaris. Concerning non-target effects, in standard laboratory considtions the predation efficiency of Danio rerio zebrafishes was 68.8% and 61.6% against I and II instar larvae of A. stephensi, respectively. In a Ch-AgNP-contaminated environment, fish predation was boosted to 89.5% and 77.3%, respectively. Quantitative analysis of antioxidant enzymes SOD, CAT and LPO from hepatopancreas of fresh water crabs Paratelphusa hydrodromous exposed for 16 days to a Ch-AgNP-contaminated aquatic environment were conducted. Notably, deleterious effects of Ch-AgNP contaminating aquatic enviroment on the non-target crab P. hydrodromous were observed, particularly when doses higher than 8–10 ppm are tested. Overall, this research highlights the potential of Ch-AGNP for the development of newer control tools against young instar populations of malaria mosquitoes, also highlighting some risks concerned the employ of nanoparticles in aquatic environments.
KW - Anopheles stephensi
KW - Culicidae
KW - Danio rerio
KW - Non-target effects
KW - Plasmodium
KW - biosafety
UR - http://www.scopus.com/inward/record.url?scp=84976272003&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2016.06.021
DO - 10.1016/j.ecoenv.2016.06.021
M3 - 期刊論文
C2 - 27344400
AN - SCOPUS:84976272003
SN - 0147-6513
VL - 132
SP - 318
EP - 328
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
ER -