Fabrication of a Molecularly Imprinted Nano-Interface-Based Electrochemical Biosensor for the Detection of CagA Virulence Factors of H. pylori

Kirti Saxena, Bayu Tri Murti, Po Kang Yang, Bansi Dhar Malhotra, Nidhi Chauhan, Utkarsh Jain

研究成果: 雜誌貢獻期刊論文同行評審

8 引文 斯高帕斯(Scopus)

摘要

H. pylori is responsible for several stomach-related diseases including gastric cancer. The main virulence factor responsible for its establishment in human gastric cells is known as CagA. Therefore, in this study, we have fabricated a highly sensitive MIP-based electrochemical biosensor for the detection of CagA. For this, an rGO and gold-coated, screen-printed electrode sensing platform was designed to provide a surface for the immobilization of a CagA-specific, molecularly imprinted polymer; then it was characterized electrochemically. Interestingly, molecular dynamics simulations were studied to optimize the MIP prepolymerization system, resulting in a well-matched, optimized molar ratio within the experiment. A low binding energy upon template removal indicates the capability of MIP to recognize the CagA antigen through a strong binding affinity. Under the optimized electrochemical experimental conditions, the fabricated CagA-MIP/Au/rGO@SPE sensor exhibited high sensitivity (0.275 µA ng−1 mL−1) and a very low limit of detection (0.05 ng mL−1) in a linear range of 0.05–50 ng mL−1. The influence of other possible interferents in analytical response has also been observed with the successful determination of the CagA antigen.

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文章編號1066
期刊Biosensors
12
發行號12
DOIs
出版狀態已出版 - 12月 2022

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