Fabrication and characterization of polymethylmethacrylate (PMMA) thin film by plasma polymerization used for cell culture

Chuan Li, J. H. Hsieh, W. W. Hu, Y. H. Lin

研究成果: 雜誌貢獻期刊論文同行評審

5 引文 斯高帕斯(Scopus)

摘要

Poly-methyl-methacrylate thin films were deposited by plasma polymerization under different radio-frequency discharge powers, monomer partial pressures, and deposition times. Surface profiler was used to evaluate the thickness and deposition rates of deposited films; Fourier transform infrared spectroscopy for the microstructures; X-ray photoelectron spectroscopy for the surface chemical compositions and atomic force microscope for the surface morphology. In addition to the as-deposited PMMA films, some selected films were further treated by oxygen to modify their surfaces. After thorough material characterizations, the films were used as substrates to culture human hepatocytes for testing their biocompatibility. Results of the cell culture were assessed by trypsin cell count, ELISA and MTT assay. It is found that the human hepatocytes can proliferate and perform urea synthesis well on the deposited poly-methyl-methacrylate thin films with or without oxygen treatment.

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頁(從 - 到)20-26
頁數7
期刊Surface and Coatings Technology
259
發行號PA
DOIs
出版狀態已出版 - 25 11月 2014

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