Fabrication and application of amperometric glucose biosensor based on a novel PtPd bimetallic nanoparticle decorated multi-walled carbon nanotube catalyst

Kuan Jung Chen, Chia Feng Lee, John Rick, Shih Han Wang, Chung Chiun Liu, Bing Joe Hwang

研究成果: 雜誌貢獻期刊論文同行評審

133 引文 斯高帕斯(Scopus)

摘要

A sensitive, selective and stable amperometric glucose biosensor employing novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) was investigated. PtPd-MWCNTs were prepared by a modified Watanabe method, and characterized by XRD and TEM. The biosensor was constructed by immobilizing the PtPd-MWCNTs catalysts in a Nafion film on a glassy carbon electrode. An inner Nafi{ligature}on film coating was used to eliminate common interferents such as uric acid, ascorbic acid and fructose. Finally, a highly porous surface with an orderly three-dimensional network enzyme layer (CS-GA-GOx) was fabricated by electrodeposition. The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062-14.07mM) and a low detection limit 0.031mM. The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM -1cm -2). The Michaelis-Menten constant (K m) was determined as 3.3mM. In addition, the biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability. The applicability of the biosensor to actual serum sample analysis was also evaluated.

原文???core.languages.en_GB???
頁(從 - 到)75-81
頁數7
期刊Biosensors and Bioelectronics
33
發行號1
DOIs
出版狀態已出版 - 15 3月 2012

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