Exploring Enzyme Encapsulation Efficiency in MOFs Using Eco-Friendly Approaches

Trung Hieu Vo, Shang Wei Lin, Miao Chun Lin, Pu Yun Kuan, Jing Hui Chen, Hsin Kai Huang, Wen-Tzu Liu, Hui Xu, Ao Li, Yu An Hsu, Lei Wan, Phuc Khanh Lam, Lien Yang Chou, Hsiao Ching Yang, Fa Kuen Shieh

研究成果: 雜誌貢獻期刊論文同行評審

摘要

The encapsulation of protein enzymes in metal-organic frameworks (MOFs) has been recognized as an effective enzyme immobilization approach. In this study, we demonstrated the influence of enzyme amount and the isoelectric points (pI) of different enzymes on the enzyme loading capacity in both mechanochemical (ball-milling) and water-based approaches. We found that increasing enzyme amounts enhances MOF enzyme loading without compromising activity, while the MOF shell protects encapsulated enzymes from proteinase K degradation through its size-sheltering mechanism. However, an excess of enzymes can hinder the formation of ZIF-90. Moreover, enzymes with low pI values (e. g., catalase, pI 5.4) facilitate encapsulation in MOFs, whereas enzymes with high pI values (e. g., lysozyme, pI 11.35) are more challenging to encapsulate. The simulation results revealed that increasing the enzyme amounts and pI values raises the activation energy necessary for MOF formation. This study highlights the crucial role of enzyme properties in the encapsulation process within MOFs, providing valuable insights for fabricating enzyme-MOF biocomposites for diverse applications, such as protein drug delivery.

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期刊ChemSusChem
DOIs
出版狀態已被接受 - 2024

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