Enzymatic determination of diglyceride using an iridium nano-particle based single use, disposable biosensor

Shu Yi Hsu, Brandon Bartling, Christina Wang, Fuh Sheng Shieu, Chung Chiun Liu

研究成果: 雜誌貢獻期刊論文同行評審

4 引文 斯高帕斯(Scopus)

摘要

A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media.

原文???core.languages.en_GB???
頁(從 - 到)5758-5773
頁數16
期刊Sensors (Switzerland)
10
發行號6
DOIs
出版狀態已出版 - 6月 2010

指紋

深入研究「Enzymatic determination of diglyceride using an iridium nano-particle based single use, disposable biosensor」主題。共同形成了獨特的指紋。

引用此