Electrochemical evaluation of lactate dehydrogenase immobilized in polyacrylamide gels

A. K. Chen, C. C. Liu

研究成果: 雜誌貢獻期刊論文同行評審

7 引文 斯高帕斯(Scopus)

摘要

Lactate dehydrogenase (EC 1.1.1.27) has been immobilized in polyacrylamide gels over a platinum grid matrix. The immobilized enzyme is used to oxidize L‐lactate in the presence of nicotinamide adenine dinucleotide (NAD+) and femcyanide. The NADH produced is then chemically oxidized back to NAD+by ferricyanide. The coupled reduction of ferricyanide ions to ferrocyanide ions results in a measurable electrochemical potential. This measurable zero‐current potential is found to be Nernstian in nature and directly proportional to the logarithm values of L‐Iactate concentration over the range of 2 × 10−5to 5 × 10−2M. The results indicate that immobilized lactate dehydrogenase can be incorporated into a system to detect L‐Lactate acid in aqueous solutions.

原文???core.languages.en_GB???
頁(從 - 到)1785-1792
頁數8
期刊Biotechnology and Bioengineering
19
發行號12
DOIs
出版狀態已出版 - 12月 1977

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