Effect of Temperature on Hydrophobie Interaction between Proteins and Hydrophobic Adsorbents: Studies by Isothermal Titration Calorimetry and the van't Hoff Equation

Wen Yih Chen, Hsiang Ming Huang, Chien Chen Lin, Fu Yung Lin, Yu Chia Chan

研究成果: 雜誌貢獻期刊論文同行評審

117 引文 斯高帕斯(Scopus)

摘要

This study investigated the binding mechanism between proteins and hydrophobia adsorbents from a thermodynamics aspect. The proteins investigated here included lysozyme, myoglobin, and α-amylase. The binding isotherms were obtained for the proteins with butyl-sepharose and octyl-sepharose resins at various temperatures and salt concentrations. The binding isotherms were then analyzed using the van't Hoff equation, and the binding enthalpy and entropy of each step of the binding process are discussed. Moreover, the effects of ligand chain length and protein characteristics were also investigated. Notably, the preferential interaction model has been adopted in the discussion of the dehydration step in the binding mechanism. The analytical results show that the binding process is entropy- dominated at higher temperature and the amount of released water molecules increases with temperature. Furthermore, the binding enthalpy (ΔHVH) and heat capacity were calculated by the van't Hoff and Kirchoff equations and were compared with the directly measured enthalpy (ΔHITC) by isothermal titration calorimetry. The analytical results provide useful insights into hydrophobic interaction in biorecognition systems.

原文???core.languages.en_GB???
頁(從 - 到)9395-9403
頁數9
期刊Langmuir
19
發行號22
DOIs
出版狀態已出版 - 28 10月 2003

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