DNA methylation profiles in monozygotic and dizygotic twins

Zachary A. Kaminsky; Thomas Tang; Sun Chong Wang; Carolyn Ptak; Gabriel H.T. Oh; Albert H.C. Wong; Laura A. Feldcamp; Carl Virtanen; Jonas Halfvarson; Curt Tysk; Allan F. McRae; Peter M. Visscher; Grant W. Montgomery; Irving I. Gottesman; Nicholas G. Martin; Art Petronis

doi:10.1038/ng.286

DNA methylation profiles in monozygotic and dizygotic twins

Zachary A. Kaminsky, Thomas Tang, Sun Chong Wang, Carolyn Ptak, Gabriel H.T. Oh, Albert H.C. Wong, Laura A. Feldcamp, Carl Virtanen, Jonas Halfvarson, Curt Tysk, Allan F. McRae, Peter M. Visscher, Grant W. Montgomery, Irving I. Gottesman, Nicholas G. Martin, Art Petronis

生醫科學與工程學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

570 引文斯高帕斯（Scopus）

摘要

Twin studies have provided the basis for genetic and epidemiological studies in human complex traits. As epigenetic factors can contribute to phenotypic outcomes, we conducted a DNA methylation analysis in white blood cells (WBC), buccal epithelial cells and gut biopsies of 114 monozygotic (MZ) twins as well as WBC and buccal epithelial cells of 80 dizygotic (DZ) twins using 12K CpG island microarrays. Here we provide the first annotation of epigenetic metastability of ∼6,000 unique genomic regions in MZ twins. An intraclass correlation (ICC)-based comparison of matched MZ and DZ twins showed significantly higher epigenetic difference in buccal cells of DZ co-twins (P = 1.2 × 10^-294). Although such higher epigenetic discordance in DZ twins can result from DNA sequence differences, our in silico SNP analyses and animal studies favor the hypothesis that it is due to epigenomic differences in the zygotes, suggesting that molecular mechanisms of heritability may not be limited to DNA sequence differences.

原文	???core.languages.en_GB???
頁（從 - 到）	240-245
頁數	6
期刊	Nature Genetics
卷	41
發行號	2
DOIs	https://doi.org/10.1038/ng.286
出版狀態	已出版 - 2月 2009

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10.1038/ng.286

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abstract = "Twin studies have provided the basis for genetic and epidemiological studies in human complex traits. As epigenetic factors can contribute to phenotypic outcomes, we conducted a DNA methylation analysis in white blood cells (WBC), buccal epithelial cells and gut biopsies of 114 monozygotic (MZ) twins as well as WBC and buccal epithelial cells of 80 dizygotic (DZ) twins using 12K CpG island microarrays. Here we provide the first annotation of epigenetic metastability of ∼6,000 unique genomic regions in MZ twins. An intraclass correlation (ICC)-based comparison of matched MZ and DZ twins showed significantly higher epigenetic difference in buccal cells of DZ co-twins (P = 1.2 × 10-294). Although such higher epigenetic discordance in DZ twins can result from DNA sequence differences, our in silico SNP analyses and animal studies favor the hypothesis that it is due to epigenomic differences in the zygotes, suggesting that molecular mechanisms of heritability may not be limited to DNA sequence differences.",

author = "Kaminsky, {Zachary A.} and Thomas Tang and Wang, {Sun Chong} and Carolyn Ptak and Oh, {Gabriel H.T.} and Wong, {Albert H.C.} and Feldcamp, {Laura A.} and Carl Virtanen and Jonas Halfvarson and Curt Tysk and McRae, {Allan F.} and Visscher, {Peter M.} and Montgomery, {Grant W.} and Gottesman, {Irving I.} and Martin, {Nicholas G.} and Art Petronis",

note = "Funding Information: This paper is dedicated to the memory of Professor V.M. Gindilis, an excellent scientist, dedicated teacher and creative twin researcher. We should like to thank S. Ziegler for technical assistance, A. Henders and M. Campbell for selection and preparation of twin DNA samples, and J. Chow for work generating the karyograms. This project was supported by the National Institute of Mental Health (R01 MH074127-01), the Canadian Institutes for Health and Research (CIHR) and the National Alliance for Research on Schizophrenia and Depression (NARSAD). A.P. is Senior Fellow of the Ontario Mental Health Foundation. Z.A.K. was supported by a CIHR graduate fellowship.",

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AU - Wong, Albert H.C.

AU - Feldcamp, Laura A.

AU - Virtanen, Carl

AU - Halfvarson, Jonas

AU - Tysk, Curt

AU - McRae, Allan F.

AU - Visscher, Peter M.

AU - Montgomery, Grant W.

AU - Gottesman, Irving I.

AU - Martin, Nicholas G.

AU - Petronis, Art

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DNA methylation profiles in monozygotic and dizygotic twins

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