Development of an IoT-integrated digital droplet nucleic acid detection system for liquid biopsy screening

Yi Chien Chen, Yu He Liu, Zhi Yang Wei, Li Hsien Ho, I. Hsuan Chou, Yun Hsien Chung, Tao Yun Yen, Wei Chun Lan, Hsien Wen Yao, Peng Wei Hsu, Chen Han Huang, Hsing Ying Lin

研究成果: 書貢獻/報告類型會議論文篇章同行評審


Glioblastoma (GBM) is the most aggressive malignant primary brain tumor[1]. Effective treatments on the early-stage glioblastoma may improve its poor survival rate and poor prognosis[2]. In this pilot study, a low copy number of nucleic acid detection system using a high-throughput microfluidic droplet imaging device was developed to achieve the specific nucleic acid detection of extracellular vesicles (EVs). Our aim is to realize an inexpensive liquid biopsy tool for the early detection of GBM using human plasma and cerebrospinal fluid (CSF). Our results demonstrate that: (a) the fabricated microfluidic chip (10 mm × 25 mm) can produce more than 25,000 droplets within 3 minutes. The average volume of generated droplets is 0.043 nanoliter (nL); (b) the entire droplet image can be acquired within 15 seconds without using the conventional stitching technique; (c) additionally, we automated the droplet image processing to determine the absolute copy number and concentration of nucleic acid samples. This portable IoT-integrated digital droplet nucleic acid sensing system provide an inexpensive and affordable optical biopsy screening tool for the early diagnosis and treatment surveillance of brain tumors. Our work can be applied into other diseases, such as colorectal cancer, pancreatic cancer and breast cancer.

主出版物標題Biomedical Imaging and Sensing Conference
編輯Toyohiko Yatagai
出版狀態已出版 - 2023
事件Biomedical Imaging and Sensing Conference 2023 - Yokohama, Japan
持續時間: 17 4月 202321 4月 2023


名字Proceedings of SPIE - The International Society for Optical Engineering


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