Development of an image profiling method for high- throughput single extracellular vesicle analysis

Li Hsien Ho, Zhi Yang Wei, Yi Chien Chen, Yu He Liu, Chia Hung Chen, Tao Yun Yen, Wei Chun Lan, Yun Hsien Chung, I. Hsuan Chou, Hsien Wen Yao, Peng Wei Hsu, Chen Han Huang, Hsing Ying Lin

研究成果: 書貢獻/報告類型會議論文篇章同行評審


5-ALA (5-aminolevulinic acid) administration for positioning tumor tissues in craniotomy is growing since the FDA approval has already passed in June 2017 [1]. This may also open a path to the convenient single extracellular vesicle (EV) analysis in liquid biopsy for tracking and analyzing the tumor-derived EVs with PPIX expression, the middle byproduct upon the oral intake of 5-ALA. 5-ALA-based fluorescence is due to preferential accumulation of the fluorophore protoporphyrin-IX (PpIX) in the malignant glioma tissue. Single EV analysis has become technically feasible and promising in studies of early cancer diagnosis and clinical translational medicine applications [2]. It can minimize the heterogenous issue between EVs once capturing a group of low percentage biomarker-positive EVs. There is a need to scale up and implement an easy-to-use single EV analysis platform to be organized into the routine practices of current research and clinical laboratories to enable early cancer diagnostics. We are combining the 5-ALA dyed EV samples with single EV detection method to establish a more efficient and precise approach for patient’s early cancer diagnosis.

主出版物標題Biomedical Imaging and Sensing Conference
編輯Toyohiko Yatagai
出版狀態已出版 - 2023
事件Biomedical Imaging and Sensing Conference 2023 - Yokohama, Japan
持續時間: 17 4月 202321 4月 2023


名字Proceedings of SPIE - The International Society for Optical Engineering


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