TY - JOUR
T1 - Determination of chronological aging parameters in epidermal keratinocytes by in vivo harmonic generation microscopy
AU - Liao, Yi Hua
AU - Chen, Szu Yu
AU - Chou, Sin Yo
AU - Wang, Pei Hsun
AU - Tsai, Ming Rung
AU - Sun, Chi Kuang
PY - 2013
Y1 - 2013
N2 - Skin aging is an important issue in geriatric and cosmetic dermatology. To quantitatively analyze changes in keratinocytes related to intrinsic aging, we exploited a 1230 nm-based in vivo harmonic generation microscopy, combining second- and third-harmonic generation modalities. 52 individuals (21 men and 31 women, age range 19-79) were examined on the sun-protected volar forearm. Through quantitative analysis by the standard algorithm provided, we found that the cellular and nuclear size of basal keratinocytes, but not that of granular cells, was significantly increased with advancing age. The cellular and nuclear areas, which have an increase of 0.51 μm2 and 0.15 μm2 per year, respectively, can serve as scoring indices for intrinsic skin aging.
AB - Skin aging is an important issue in geriatric and cosmetic dermatology. To quantitatively analyze changes in keratinocytes related to intrinsic aging, we exploited a 1230 nm-based in vivo harmonic generation microscopy, combining second- and third-harmonic generation modalities. 52 individuals (21 men and 31 women, age range 19-79) were examined on the sun-protected volar forearm. Through quantitative analysis by the standard algorithm provided, we found that the cellular and nuclear size of basal keratinocytes, but not that of granular cells, was significantly increased with advancing age. The cellular and nuclear areas, which have an increase of 0.51 μm2 and 0.15 μm2 per year, respectively, can serve as scoring indices for intrinsic skin aging.
UR - http://www.scopus.com/inward/record.url?scp=84871765485&partnerID=8YFLogxK
U2 - 10.1364/BOE.4.000077
DO - 10.1364/BOE.4.000077
M3 - 期刊論文
C2 - 23304649
AN - SCOPUS:84871765485
SN - 2156-7085
VL - 4
SP - 77
EP - 88
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 1
ER -