Determination of chronological aging parameters in epidermal keratinocytes by in vivo harmonic generation microscopy

Yi Hua Liao, Szu Yu Chen, Sin Yo Chou, Pei Hsun Wang, Ming Rung Tsai, Chi Kuang Sun

研究成果: 雜誌貢獻期刊論文同行評審

33 引文 斯高帕斯(Scopus)

摘要

Skin aging is an important issue in geriatric and cosmetic dermatology. To quantitatively analyze changes in keratinocytes related to intrinsic aging, we exploited a 1230 nm-based in vivo harmonic generation microscopy, combining second- and third-harmonic generation modalities. 52 individuals (21 men and 31 women, age range 19-79) were examined on the sun-protected volar forearm. Through quantitative analysis by the standard algorithm provided, we found that the cellular and nuclear size of basal keratinocytes, but not that of granular cells, was significantly increased with advancing age. The cellular and nuclear areas, which have an increase of 0.51 μm2 and 0.15 μm2 per year, respectively, can serve as scoring indices for intrinsic skin aging.

原文???core.languages.en_GB???
頁(從 - 到)77-88
頁數12
期刊Biomedical Optics Express
4
發行號1
DOIs
出版狀態已出版 - 2013

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