Determination of alanine aminotransferase with an electrochemical nano Ir-C biosensor for the screening of liver diseases

Chang Jung Hsueh, Joanne H. Wang, Liming Dai, Chung Chiun Liu

研究成果: 雜誌貢獻期刊論文同行評審

27 引文 斯高帕斯(Scopus)

摘要

Alanine aminotransaminase (ALT), is an enzyme that normally resides in serum and body tissues, especially in the liver. It is released into the serum as a result of tissue injury; hence the concentration of ALT in the serum may be increased with acute damage to hepatic cells. A single use, disposable biosensor, comprising iridium nano-particle as catalyst dispersed on carbon paste, has been developed for the determination of ALT concentration. The biosensor is based on quantifying H2O2 concentration produced by a serial of ALT enzymatic reactions. It operates well at room temperature in different physiological fluids: phosphate buffer, calf serum and human serum for ALT concentration of 0-544 ng/mL. Experimental results in human serum are compared to those obtained by spectrophotometric assays with excellent agreement. Therefore, the Ir/C biosensor shows good relationship on the dilution of concentrated ALT clinical applications.

原文???core.languages.en_GB???
頁(從 - 到)107-117
頁數11
期刊Biosensors
1
發行號3
DOIs
出版狀態已出版 - 9月 2011

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