Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors

You Ren Hsu; Geng Yen Lee; Jen Inn Chyi; Chung Ke Chang; Chih Cheng Huang; Chen Pin Hsu; Tai Huang Huang; Fan Ren; Yu Lin Wang

doi:10.1149/05006.0239ecst

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors

You Ren Hsu, Geng Yen Lee, Jen Inn Chyi, Chung Ke Chang, Chih Cheng Huang, Chen Pin Hsu, Tai Huang Huang, Fan Ren, Yu Lin Wang

研究成果: 書貢獻/報告類型 › 會議論文篇章 › 同行評審

13 引文斯高帕斯（Scopus）

摘要

AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotopelabeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-protein interaction.

原文	???core.languages.en_GB???
主出版物標題	Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012
發行者	Electrochemical Society Inc.
頁面	239-243
頁數	5
版本	6
ISBN（列印）	9781607683544
DOIs	https://doi.org/10.1149/05006.0239ecst
出版狀態	已出版 - 2013
事件	Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012 - Honolulu, HI, United States 持續時間: 7 10月 2012 → 12 10月 2012

出版系列

名字	ECS Transactions
號碼	6
卷	50
ISSN（列印）	1938-5862
ISSN（電子）	1938-6737

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???event.eventtypes.event.conference???	Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012
國家/地區	United States
城市	Honolulu, HI
期間	7/10/12 → 12/10/12

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10.1149/05006.0239ecst

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Hsu, Y. R., Lee, G. Y., Chyi, J. I., Chang, C. K., Huang, C. C., Hsu, C. P., Huang, T. H., Ren, F., & Wang, Y. L. (2013). Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. 於 Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012 (6 編輯, 頁 239-243). (ECS Transactions; 卷 50, 編號 6). Electrochemical Society Inc.. https://doi.org/10.1149/05006.0239ecst

Hsu, You Ren ; Lee, Geng Yen ; Chyi, Jen Inn 等. / Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6. 編輯 Electrochemical Society Inc., 2013. 頁 239-243 (ECS Transactions; 6).

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title = "Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors",

abstract = "AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotopelabeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-protein interaction.",

author = "Hsu, {You Ren} and Lee, {Geng Yen} and Chyi, {Jen Inn} and Chang, {Chung Ke} and Huang, {Chih Cheng} and Hsu, {Chen Pin} and Huang, {Tai Huang} and Fan Ren and Wang, {Yu Lin}",

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note = "Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012 ; Conference date: 07-10-2012 Through 12-10-2012",

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Hsu, YR, Lee, GY, Chyi, JI, Chang, CK, Huang, CC, Hsu, CP, Huang, TH, Ren, F & Wang, YL 2013, Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. 於 Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6 edn, ECS Transactions, 編號 6, 卷 50, Electrochemical Society Inc., 頁 239-243, Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012, Honolulu, HI, United States, 7/10/12. https://doi.org/10.1149/05006.0239ecst

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. / Hsu, You Ren; Lee, Geng Yen; Chyi, Jen Inn 等.
Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6. 編輯 Electrochemical Society Inc., 2013. p. 239-243 (ECS Transactions; 卷 50, 編號 6).

研究成果: 書貢獻/報告類型 › 會議論文篇章 › 同行評審

TY - GEN

T1 - Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors

AU - Hsu, You Ren

AU - Lee, Geng Yen

AU - Chyi, Jen Inn

AU - Chang, Chung Ke

AU - Huang, Chih Cheng

AU - Hsu, Chen Pin

AU - Huang, Tai Huang

AU - Ren, Fan

AU - Wang, Yu Lin

PY - 2013

Y1 - 2013

N2 - AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotopelabeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-protein interaction.

AB - AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotopelabeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-protein interaction.

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BT - Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012

PB - Electrochemical Society Inc.

T2 - Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012

Y2 - 7 October 2012 through 12 October 2012

ER -

Hsu YR, Lee GY, Chyi JI, Chang CK, Huang CC, Hsu CP 等. Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors. 於 Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6 編輯 Electrochemical Society Inc. 2013. p. 239-243. (ECS Transactions; 6). doi: 10.1149/05006.0239ecst

Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein using AlGaN/GaN high electron mobility transistors

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