Detection of adenosine deaminase activity with a thick-film screen-printed Ir/C sensor to detect liver disease

Brandon Bartling, Lu Li, Chung Chiun Liu

研究成果: 雜誌貢獻期刊論文同行評審

1 引文 斯高帕斯(Scopus)

摘要

An electrochemical biosensor, comprising a thick-film screen-printed Ir/C working and counter electrodes, was developed for the detection of the enzymatic activity of adenosine deaminase for potential patient management with liver disease. Present methods of detecting adenosine deaminase involve spectrometric methods that require costly, nonportable equipment that is ill-suited for a point-of-care testing. Studies dealing with the electrochemical detection of adenosine deaminase intermediates such as xanthine and hypoxanthine required the use of +0.5 V, vs a Ag/AgCl reference electrode, and a large sample volume, 1 mL or more. The proposed biosensor used a sample volume of 3 μL and a potential of +0.27 V vs Ag/AgCl. This biosensor used the enzymes xanthine oxidase (EC 1.1.3.22) and purine nucleoside phosphorylase (EC 2.4.2.1) coimmobilized on the thick-film screen-printed working electrode to detect enzymatically generated H2 O2. Constant potential measurements showed that this biosensor had good linear performance over a 0-36 units/L of adenosine deaminase concentration range in phosphate buffer, mouse plasma, and mouse whole blood. The effect of the variation in the quantity of enzyme on the current output and the interference from intermediate-formed compounds were also explored.

原文???core.languages.en_GB???
頁(從 - 到)J130-J134
期刊Journal of the Electrochemical Society
157
發行號4
DOIs
出版狀態已出版 - 2010

指紋

深入研究「Detection of adenosine deaminase activity with a thick-film screen-printed Ir/C sensor to detect liver disease」主題。共同形成了獨特的指紋。

引用此