TY - JOUR
T1 - Derivatization procedures and determination of levoglucosan and related monosaccharide anhydrides in atmospheric aerosols by gas chromatography-mass spectrometry
AU - Hsu, Ching Lin
AU - Cheng, Chin Yuan
AU - Lee, Chung Te
AU - Ding, Wang Hsien
N1 - Funding Information:
The authors would like to thank the National Science Council of Taiwan for financially supporting this research under contract No. 94-2113-M-008-013.
PY - 2007/4/15
Y1 - 2007/4/15
N2 - This study evaluated the derivatization procedures for detecting the three most commonly monosaccharide anhydrides (MAs) (levoglucosan, mannosan and galactosan) in atmospheric aerosols using gas chromatography-mass spectrometry (GC-MS). Various silylating agents, mainly trimethylsilylating agents (TMS), were compared and the effects of various contents of trimethylchlorosilane (TMCS, as a stimulator) were evaluated to optimize the conditions for detecting these compounds in aerosol samples. Differences among the abundances of the derivatives were caused by the sterical hindrance of three hydroxyl groups in the structures of monosaccharide anhydrides. The effects of the reaction time and temperature were also examined. The optimal reaction time and temperature were 60 min and 80 °C with 1% TMCS plus 0.2% 1,4-dithioerythritol (DTE). Under these conditions, the percentages of formation of bis-O-TMS derivatives (as by-products) were 23, 29 and 10% for galactosan, mannosan and levoglucosan, respectively. The concentrations of galactosan, mannosan and levoglucosan in particles of smoke samples ranged from 29 to 88, 23 to 69 and 77 to 380 ng/m3, respectively; and in particles of atmospheric aerosols ranged from 0.06 to 0.75, n.d. to 0.49 and 1.6 to 132 ng/m3, respectively. Levoglucosan was the dominant MAs detected in both type of samples. Less than 10% quantitation difference was obtained when bis-O-TMS derivatives were included in the calculation.
AB - This study evaluated the derivatization procedures for detecting the three most commonly monosaccharide anhydrides (MAs) (levoglucosan, mannosan and galactosan) in atmospheric aerosols using gas chromatography-mass spectrometry (GC-MS). Various silylating agents, mainly trimethylsilylating agents (TMS), were compared and the effects of various contents of trimethylchlorosilane (TMCS, as a stimulator) were evaluated to optimize the conditions for detecting these compounds in aerosol samples. Differences among the abundances of the derivatives were caused by the sterical hindrance of three hydroxyl groups in the structures of monosaccharide anhydrides. The effects of the reaction time and temperature were also examined. The optimal reaction time and temperature were 60 min and 80 °C with 1% TMCS plus 0.2% 1,4-dithioerythritol (DTE). Under these conditions, the percentages of formation of bis-O-TMS derivatives (as by-products) were 23, 29 and 10% for galactosan, mannosan and levoglucosan, respectively. The concentrations of galactosan, mannosan and levoglucosan in particles of smoke samples ranged from 29 to 88, 23 to 69 and 77 to 380 ng/m3, respectively; and in particles of atmospheric aerosols ranged from 0.06 to 0.75, n.d. to 0.49 and 1.6 to 132 ng/m3, respectively. Levoglucosan was the dominant MAs detected in both type of samples. Less than 10% quantitation difference was obtained when bis-O-TMS derivatives were included in the calculation.
KW - Aerosols
KW - Derivatization
KW - GC-MS
KW - Monosaccharide anhydrides
UR - http://www.scopus.com/inward/record.url?scp=33947317598&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2006.10.018
DO - 10.1016/j.talanta.2006.10.018
M3 - 期刊論文
C2 - 19071602
AN - SCOPUS:33947317598
SN - 0039-9140
VL - 72
SP - 199
EP - 205
JO - Talanta
JF - Talanta
IS - 1
ER -