Comparison of Escherichia coli surface attachment methods for single-cell microscopy

Yao Kuan Wang, Ekaterina Krasnopeeva, Ssu Yuan Lin, Fan Bai, Teuta Pilizota, Chien Jung Lo

研究成果: 雜誌貢獻期刊論文同行評審

4 引文 斯高帕斯(Scopus)

摘要

For in vivo, single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial physiology. However, a systematic comparison of the effects these attachment methods have on the bacterial physiology is lacking. Here we present such a comparison for bacterium Escherichia coli, and assess the growth rate, size and intracellular pH of cells growing attached to different, commonly used, surfaces. We demonstrate that E. coli grow at the same rate, length and internal pH on all the tested surfaces when in the same growth medium. The result suggests that tested attachment methods can be used interchangeably when studying E. coli physiology.

原文???core.languages.en_GB???
文章編號19418
期刊Scientific Reports
9
發行號1
DOIs
出版狀態已出版 - 1 12月 2019

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