Commensal staphylococcus aureus provokes immunity to protect against skin infection of methicillin-resistant staphylococcus aureus

John Jackson Yang, Ting Wei Chang, Yong Jiang, Hsin Jou Kao, Bin Hao Chiou, Ming Shan Kao, Chun Ming Huang

研究成果: 雜誌貢獻期刊論文同行評審

22 引文 斯高帕斯(Scopus)

摘要

Unlike USA300, a strain of community-acquired methicillin-resistant Staphylococcus aureus (MRSA), commensal Staphylococcus aureus (S. aureus) bacteria isolated from human skin demonstrated the ability to mediate the glycerol fermentation to produce short-chain fatty acids (SCFAs). Quantitative proteomic analysis of enzymes involved in glycerol fermentation demonstrated that the expression levels of six enzymes, including glycerol-3-phosphate dehydrogenase (GPDH) and phosphoglycerate mutase (PGM), in commensal S. aureus are more than three-fold higher than those in USA300. Western blotting validated the low expression levels of GPDH in USA300, MRSA252 (a strain of hospital-acquired MRSA), and invasive methicillin-susceptible S. aureus (MSSA). In the presence of glycerol, commensal S. aureus effectively suppressed the growth of USA300 in vitro and in vivo. Active immunization of mice with lysates or recombinant α-hemolysin of commensal S. aureus or passive immunization with neutralizing sera provided immune protection against the skin infection of USA300. Our data illustrate for the first time that commensal S. aureus elicits both innate and adaptive immunity via glycerol fermentation and systemic antibody production, respectively, to fight off the skin infection of pathogenic MRSA.

原文???core.languages.en_GB???
文章編號1290
期刊International Journal of Molecular Sciences
19
發行號5
DOIs
出版狀態已出版 - 5月 2018

指紋

深入研究「Commensal staphylococcus aureus provokes immunity to protect against skin infection of methicillin-resistant staphylococcus aureus」主題。共同形成了獨特的指紋。

引用此