Catalysis of rice straw hydrolysis by the combination of immobilized cellulase from aspergillus niger on β -Cyclodextrin-Fenanoparticles and ionic liquid

Po Jung Huang, Ken Lin Chang, Jung Feng Hsieh, Shui Tein Chen

研究成果: 雜誌貢獻期刊論文同行評審

51 引文 斯高帕斯(Scopus)

摘要

Cellulase from Aspergillus niger was immobilized onto β-cyclodextrin-conjugated magnetic particles by silanization and reductive amidation. The immobilized cellulase gained supermagnetism due to the magnetic nanoparticles. Ninety percent of cellulase was immobilized, but the activity of immobilized cellulase decreased by 10%. In this study, ionic liquid (1-butyl-3-methylimidazolium chloride) was introduced into the hydrolytic process because the original reaction was a solid-solid reaction. The activity of immobilized cellulase was improved from 54.87 to 59.11 U g immobilized cellulase-1 at an ionic liquid concentration of 200 mM. Using immobilized cellulase and ionic liquid in the hydrolysis of rice straw, the initial reaction rate was increased from 1.629 to 2.739 g h-1 L-1. One of the advantages of immobilized cellulase is high reusability - it was usable for a total of 16 times in this study. Compared with free cellulase, magnetized cellulase can be recycled by magnetic field and the activity of immobilized cellulase was shown to remain at 85% of free cellulase without denaturation under a high concentration of glucose (15 g L-1). Therefore, immobilized cellulase can hydrolyze rice straw continuously compared with free cellulase. The amount of harvested glucose can be up to twentyfold higher than that from the hydrolysis by free cellulase.

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文章編號409103
期刊BioMed Research International
2015
DOIs
出版狀態已出版 - 2015

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