TY - JOUR
T1 - Burkholderia pseudomallei infection induces the expression of apoptosis-related genes and proteins in mouse macrophages
AU - Hseu, You Cheng
AU - Sung, Jia Chuen
AU - Shieh, Bao Sen
AU - Chen, Ssu C.hing
N1 - Publisher Copyright:
Copyright © 2013. Published by Elsevier B.V.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - BACKGROUND/PURPOSE: In this study, we addressed whether the production of apoptosis-related genes and proteins is induced in mouse macrophages infected with Burkholderia pseudomallei cells.METHODS: Mouse macrophages were infected with B. pseudomallei cells at 0.5 hours, 1 hour, 2 hours, 4 hours, and 6 hours, respectively, followed by real-time polymerase chain reaction (PCR) array analysis. The amount of apoptosis-related proteins (caspase-3, caspase -8, caspase -9, Bax, and Bcl-2) was confirmed by Western blot.RESULTS: After infection, an increase of these proteins was observed. The expression levels of other apoptosis-related genes were also determined by PCR array. Experimental results revealed that the messenger RNA levels of tumor necrosis factor ligand (e.g., tnfsf10 and tnfrs10b) and fas were increased, whereas the expression levels of some antiapoptosis genes such as Birc5, Hells, and Bnip3 were decreased.CONCLUSION: Our study results demonstrate that the apoptosis-related genes and proteins in mouse macrophages were modulated by B. pseudomallei.
AB - BACKGROUND/PURPOSE: In this study, we addressed whether the production of apoptosis-related genes and proteins is induced in mouse macrophages infected with Burkholderia pseudomallei cells.METHODS: Mouse macrophages were infected with B. pseudomallei cells at 0.5 hours, 1 hour, 2 hours, 4 hours, and 6 hours, respectively, followed by real-time polymerase chain reaction (PCR) array analysis. The amount of apoptosis-related proteins (caspase-3, caspase -8, caspase -9, Bax, and Bcl-2) was confirmed by Western blot.RESULTS: After infection, an increase of these proteins was observed. The expression levels of other apoptosis-related genes were also determined by PCR array. Experimental results revealed that the messenger RNA levels of tumor necrosis factor ligand (e.g., tnfsf10 and tnfrs10b) and fas were increased, whereas the expression levels of some antiapoptosis genes such as Birc5, Hells, and Bnip3 were decreased.CONCLUSION: Our study results demonstrate that the apoptosis-related genes and proteins in mouse macrophages were modulated by B. pseudomallei.
KW - Apoptosis-based polymerase chain reaction array
KW - Burkholderia pseudomallei
KW - Caspases
UR - http://www.scopus.com/inward/record.url?scp=85027948847&partnerID=8YFLogxK
U2 - 10.1016/j.jmii.2013.03.011
DO - 10.1016/j.jmii.2013.03.011
M3 - 期刊論文
C2 - 23751765
AN - SCOPUS:85027948847
VL - 47
SP - 394
EP - 398
JO - Journal of Microbiology, Immunology and Infection
JF - Journal of Microbiology, Immunology and Infection
SN - 1684-1182
IS - 5
ER -
