Biosensor platform based on surface plasmon-enhanced fluorescence spectroscopy and responsive hydrogel binding matrix

Chun Jen Huang, Ulrich Jonas, Jakub Dostálek, Wolfgang Knoll

研究成果: 書貢獻/報告類型會議論文篇章同行評審

9 引文 斯高帕斯(Scopus)

摘要

We report a novel biosensor platform based on surface plasmon-enhanced fluorescence spectroscopy (SPFS) and a responsive N-isopropylacrylamide (NIPAAm) hydrogel binding matrix. This binding matrix highly swells in aqueous environment and it can be modified with receptor biomolecules by using active ester coupling chemistry. After the binding of target analyte molecules contained in a sample by receptor biomolecules immobilized in the hydrogel matrix, the captured analyte molecules can be compacted on the surface through the collapse of the gel triggered by an external stimulus. A thin hydrogel NIPAAm-based film was attached to a gold sensor surface and modified with mouse IgG receptor molecules. The affinity binding of antibodies against mouse IgG that were labeled with Alexa Fluor chromophores was observed by surface plasmon-enhanced fluorescence spectroscopy. We demonstrate that the collapse of the hydrogel matrix results in the enhancement of measured fluorescence intensity owing to the increase in the concentration of captured molecules within the evanescent field of surface plasmons.

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主出版物標題Optical Sensors 2009
DOIs
出版狀態已出版 - 2009
事件Optical Sensors 2009 - Prague, Czech Republic
持續時間: 20 4月 200922 4月 2009

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
7356
ISSN(列印)0277-786X

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???event.eventtypes.event.conference???Optical Sensors 2009
國家/地區Czech Republic
城市Prague
期間20/04/0922/04/09

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