Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section

Tsung Han Tsai; Cheng Yung Lin; Huai Jen Tsai; Szu Yu Chen; Shih Peng Tai; Kung Hsuan Lin; Chi Kuang Sun

doi:10.1364/OL.31.000930

Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section

Tsung Han Tsai, Cheng Yung Lin, Huai Jen Tsai, Szu Yu Chen, Shih Peng Tai, Kung Hsuan Lin, Chi Kuang Sun

光電科學與工程學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

27 引文斯高帕斯（Scopus）

摘要

The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wave-length in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.

原文	???core.languages.en_GB???
頁（從 - 到）	930-932
頁數	3
期刊	Optics Letters
卷	31
發行號	7
DOIs	https://doi.org/10.1364/OL.31.000930
出版狀態	已出版 - 1 4月 2006

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10.1364/OL.31.000930

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AU - Tsai, Tsung Han

AU - Lin, Cheng Yung

AU - Tsai, Huai Jen

AU - Chen, Szu Yu

AU - Tai, Shih Peng

AU - Lin, Kung Hsuan

AU - Sun, Chi Kuang

PY - 2006/4/1

Y1 - 2006/4/1

N2 - The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wave-length in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.

AB - The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wave-length in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.

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JO - Optics Letters

JF - Optics Letters

IS - 7

ER -

Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section

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