Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis

J. S. Buch; J. Liu; S. Yang; C. W. Tsao; T. Song; P. Sivanesan; K. Phalnikar; C. S. Lee; D. L. DeVoe

Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis

J. S. Buch, J. Liu, S. Yang, C. W. Tsao, T. Song, P. Sivanesan, K. Phalnikar, C. S. Lee, D. L. DeVoe

機械工程學系

研究成果: 會議貢獻類型 › 會議論文 › 同行評審

2 引文斯高帕斯（Scopus）

摘要

A fully automated multidimensional protein separation system is presented as a high-throughput alternative to traditional 2-D PAGE for comprehensive protein profiling and biomarker discovery. Here we present recent developments enabling separation of complex protein mixtures using a new chip design, a substantially modified fabrication process including the use of UV-ozone surface treatments, and in situ photopolymerization of poly(ethylene glycol)-based gel plugs to control pressure induced flow in selected regions of the chip.

原文	???core.languages.en_GB???
頁面	1579-1581
頁數	3
出版狀態	已出版 - 2006
事件	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan 持續時間: 5 11月 2006 → 9 11月 2006

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???event.eventtypes.event.conference???	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
國家/地區	Japan
城市	Tokyo
期間	5/11/06 → 9/11/06

引用此

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title = "Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis",

abstract = "A fully automated multidimensional protein separation system is presented as a high-throughput alternative to traditional 2-D PAGE for comprehensive protein profiling and biomarker discovery. Here we present recent developments enabling separation of complex protein mixtures using a new chip design, a substantially modified fabrication process including the use of UV-ozone surface treatments, and in situ photopolymerization of poly(ethylene glycol)-based gel plugs to control pressure induced flow in selected regions of the chip.",

keywords = "2-D PAGE, Gel electrophoresis, Isoelectric focusing",

author = "Buch, {J. S.} and J. Liu and S. Yang and Tsao, {C. W.} and T. Song and P. Sivanesan and K. Phalnikar and Lee, {C. S.} and DeVoe, {D. L.}",

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note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis

AU - Buch, J. S.

AU - Liu, J.

AU - Yang, S.

AU - Tsao, C. W.

AU - Song, T.

AU - Sivanesan, P.

AU - Phalnikar, K.

AU - Lee, C. S.

AU - DeVoe, D. L.

PY - 2006

Y1 - 2006

N2 - A fully automated multidimensional protein separation system is presented as a high-throughput alternative to traditional 2-D PAGE for comprehensive protein profiling and biomarker discovery. Here we present recent developments enabling separation of complex protein mixtures using a new chip design, a substantially modified fabrication process including the use of UV-ozone surface treatments, and in situ photopolymerization of poly(ethylene glycol)-based gel plugs to control pressure induced flow in selected regions of the chip.

AB - A fully automated multidimensional protein separation system is presented as a high-throughput alternative to traditional 2-D PAGE for comprehensive protein profiling and biomarker discovery. Here we present recent developments enabling separation of complex protein mixtures using a new chip design, a substantially modified fabrication process including the use of UV-ozone surface treatments, and in situ photopolymerization of poly(ethylene glycol)-based gel plugs to control pressure induced flow in selected regions of the chip.

KW - 2-D PAGE

KW - Gel electrophoresis

KW - Isoelectric focusing

UR - http://www.scopus.com/inward/record.url?scp=84902472827&partnerID=8YFLogxK

M3 - 會議論文

AN - SCOPUS:84902472827

SP - 1579

EP - 1581

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Automated two dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis

摘要

???event.eventtypes.event.conference???

指紋

引用此