Autocrine growth factor regulated mammalian cell growth on solid surfaces

Jyh Ping Hsu, Heng Kwong Tsao

研究成果: 雜誌貢獻期刊論文同行評審

摘要

We assume that adjacent cells on a solid surface may establish a microenvironment with a concentration of an autocrine growth factor (AGF) greater than a threshold value. Cells enclosed in this microenvironment undergo proliferntion as a response to the regulatory action of AGF. If no such microenvironment can be formed, cells remain unchanged on the surface. On the basis of this hypothesis, all the seemingly peculiar behaviors of the cell growth phenomenon under consideration become logical. In particular, the following problems are solved: (i) Why does there exist a critical inoculum density? (ii) Why is the critical inoculum cell number proportional to the radius of a microcarrier with a power less than two? (iii) Why is cell growth on microcarriers appreciable even at a very low level of inoculum density? (iv) Why is the inoculum density requirement for cells growing on petri dishes considerably less than that for cells growing on microcarriers? (v) Why does plating efficiency vary with inoculum density for cells growing on petri dishes, and how does it vary?

原文???core.languages.en_GB???
頁(從 - 到)125-130
頁數6
期刊Journal of the Chinese Institute of Chemical Engineers
22
發行號3
出版狀態已出版 - 5月 1991

指紋

深入研究「Autocrine growth factor regulated mammalian cell growth on solid surfaces」主題。共同形成了獨特的指紋。

引用此