Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability

Yoshihiro Fukawatase; Masashi Toyoda; Kohji Okamura; Ken Ichi Nakamura; Kazuhiko Nakabayashi; Shuji Takada; Mayu Yamazaki-Inoue; Akira Masuda; Michiyo Nasu; Kenichiro Hata; Kazunori Hanaoka; Akon Higuchi; Kaiyo Takubo; Akihiro Umezawa

doi:10.1038/srep05421

Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability

Yoshihiro Fukawatase, Masashi Toyoda, Kohji Okamura, Ken Ichi Nakamura, Kazuhiko Nakabayashi, Shuji Takada, Mayu Yamazaki-Inoue, Akira Masuda, Michiyo Nasu, Kenichiro Hata, Kazunori Hanaoka, Akon Higuchi, Kaiyo Takubo, Akihiro Umezawa

化學工程與材料工程學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

33 引文斯高帕斯（Scopus）

摘要

Ataxia telangiectasia is a neurodegenerative inherited disease with chromosomal instability and hypersensitivity to ionizing radiation. iPS cells lacking ATM (AT-iPS cells) exhibited hypersensitivity to X-ray irradiation, one of the characteristics of the disease. While parental ataxia telangiectasia cells exhibited significant chromosomal abnormalities, AT-iPS cells did not show any chromosomal instability in vitro for at least 80 passages (560 days). Whole exome analysis also showed a comparable nucleotide substitution rate in AT-iPS cells. Taken together, these data show that ATM is involved in protection from irradiation-induced cell death.

原文	???core.languages.en_GB???
文章編號	5421
期刊	Scientific Reports
卷	4
DOIs	https://doi.org/10.1038/srep05421
出版狀態	已出版 - 27 6月 2014

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10.1038/srep05421

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Fukawatase, Y., Toyoda, M., Okamura, K., Nakamura, K. I., Nakabayashi, K., Takada, S., Yamazaki-Inoue, M., Masuda, A., Nasu, M., Hata, K., Hanaoka, K., Higuchi, A., Takubo, K., & Umezawa, A. (2014). Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability. Scientific Reports, 4, [5421]. https://doi.org/10.1038/srep05421

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title = "Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability",

abstract = "Ataxia telangiectasia is a neurodegenerative inherited disease with chromosomal instability and hypersensitivity to ionizing radiation. iPS cells lacking ATM (AT-iPS cells) exhibited hypersensitivity to X-ray irradiation, one of the characteristics of the disease. While parental ataxia telangiectasia cells exhibited significant chromosomal abnormalities, AT-iPS cells did not show any chromosomal instability in vitro for at least 80 passages (560 days). Whole exome analysis also showed a comparable nucleotide substitution rate in AT-iPS cells. Taken together, these data show that ATM is involved in protection from irradiation-induced cell death.",

author = "Yoshihiro Fukawatase and Masashi Toyoda and Kohji Okamura and Nakamura, {Ken Ichi} and Kazuhiko Nakabayashi and Shuji Takada and Mayu Yamazaki-Inoue and Akira Masuda and Michiyo Nasu and Kenichiro Hata and Kazunori Hanaoka and Akon Higuchi and Kaiyo Takubo and Akihiro Umezawa",

note = "Funding Information: We would like to express our sincere thanks to M. Yamada for fruitful discussion and critical reading of the manuscript, to H. Abe and H. Kobayashi for providing expert technical assistance, to Dr. C. Ketcham for English editing and proofreading, and to E. Suzuki, Y. Kajiyama, Y. Suehiro, and K. Saito for secretarial work. This research was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan; by Ministry of Health, Labor and Welfare (MHLW) Sciences research grants; by a Research Grant on Health Science focusing on Drug Innovation from the Japan Health Science Foundation; by the program for the promotion of Fundamental Studies in Health Science of the Pharmaceuticals and Medical Devices Agency; by the Grant of National Center for Child Health and Development. We acknowledge the International High Cited Research Group (IHCRG #14-104), Deanship of Scientific Research, King Saudi University, Riyadh, Kingdom of Saudi Arabia. AU thanks King Saud University, Riyadh, Kingdom of Saudi Arabia, for the Visiting Professorship.",

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AU - Fukawatase, Yoshihiro

AU - Toyoda, Masashi

AU - Okamura, Kohji

AU - Nakamura, Ken Ichi

AU - Nakabayashi, Kazuhiko

AU - Takada, Shuji

AU - Yamazaki-Inoue, Mayu

AU - Masuda, Akira

AU - Nasu, Michiyo

AU - Hata, Kenichiro

AU - Hanaoka, Kazunori

AU - Higuchi, Akon

AU - Takubo, Kaiyo

AU - Umezawa, Akihiro

N1 - Funding Information: We would like to express our sincere thanks to M. Yamada for fruitful discussion and critical reading of the manuscript, to H. Abe and H. Kobayashi for providing expert technical assistance, to Dr. C. Ketcham for English editing and proofreading, and to E. Suzuki, Y. Kajiyama, Y. Suehiro, and K. Saito for secretarial work. This research was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan; by Ministry of Health, Labor and Welfare (MHLW) Sciences research grants; by a Research Grant on Health Science focusing on Drug Innovation from the Japan Health Science Foundation; by the program for the promotion of Fundamental Studies in Health Science of the Pharmaceuticals and Medical Devices Agency; by the Grant of National Center for Child Health and Development. We acknowledge the International High Cited Research Group (IHCRG #14-104), Deanship of Scientific Research, King Saudi University, Riyadh, Kingdom of Saudi Arabia. AU thanks King Saud University, Riyadh, Kingdom of Saudi Arabia, for the Visiting Professorship.

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N2 - Ataxia telangiectasia is a neurodegenerative inherited disease with chromosomal instability and hypersensitivity to ionizing radiation. iPS cells lacking ATM (AT-iPS cells) exhibited hypersensitivity to X-ray irradiation, one of the characteristics of the disease. While parental ataxia telangiectasia cells exhibited significant chromosomal abnormalities, AT-iPS cells did not show any chromosomal instability in vitro for at least 80 passages (560 days). Whole exome analysis also showed a comparable nucleotide substitution rate in AT-iPS cells. Taken together, these data show that ATM is involved in protection from irradiation-induced cell death.

AB - Ataxia telangiectasia is a neurodegenerative inherited disease with chromosomal instability and hypersensitivity to ionizing radiation. iPS cells lacking ATM (AT-iPS cells) exhibited hypersensitivity to X-ray irradiation, one of the characteristics of the disease. While parental ataxia telangiectasia cells exhibited significant chromosomal abnormalities, AT-iPS cells did not show any chromosomal instability in vitro for at least 80 passages (560 days). Whole exome analysis also showed a comparable nucleotide substitution rate in AT-iPS cells. Taken together, these data show that ATM is involved in protection from irradiation-induced cell death.

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Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability

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