Application of steric-mass action model to triblock copolymer as a displacer in Ion-exchange displacement chromatography

Wen Yih Chen, Kuo Che Dai, Kun Chan Ho, Fu Yung Lin, Hsiang Ming Huang, Ruoh Chyu Ruaan

研究成果: 雜誌貢獻期刊論文同行評審

1 引文 斯高帕斯(Scopus)

摘要

This study explores the feasibility of using triblock copolymer polyampholytes as a displacer to separate β-lactoglobulin A and B isoforms by anion exchange displacement chromatography. The displacers used herein are methacrylic polyampholytes: A8M12B12, A16M12B12 and A16M24B24, where A is methacrylic acid, B is 2-(dimethylamino) ethyl methacrylate, and M is methyl methacrylate. In addition, the steric factor, characteristic charges and binding equilibrium constant with the ion exchanger gel of the triblock copolymer are measured and discussed with the structure and solution behavior of the polyampholytes. With the Steric-Mass Action (SMA) model of ion-exchange displacement chromatography, the elution and displacement line can be constructed for the system. Moreover, the behavior of elution and displacement line is qualitatively discussed and successfully proven.

原文???core.languages.en_GB???
頁(從 - 到)375-382
頁數8
期刊Journal of the Chinese Institute of Chemical Engineers
30
發行號5
出版狀態已出版 - 9月 1999

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