An integrated magneto-electrochemical device for the rapid profiling of tumour extracellular vesicles from blood plasma

Jongmin Park, Jun Seok Park, Chen Han Huang, Ala Jo, Kaitlyn Cook, Rui Wang, Hsing Ying Lin, Jan Van Deun, Huiyan Li, Jouha Min, Lan Wang, Ghilsuk Yoon, Bob S. Carter, Leonora Balaj, Gyu Seog Choi, Cesar M. Castro, Ralph Weissleder, Hakho Lee

研究成果: 雜誌貢獻期刊論文同行評審

15 引文 斯高帕斯(Scopus)

摘要

Assays for cancer diagnosis via the analysis of biomarkers on circulating extracellular vesicles (EVs) typically have lengthy sample workups, limited throughput or insufficient sensitivity, or do not use clinically validated biomarkers. Here we report the development and performance of a 96-well assay that integrates the enrichment of EVs by antibody-coated magnetic beads and the electrochemical detection, in less than one hour of total assay time, of EV-bound proteins after enzymatic amplification. By using the assay with a combination of antibodies for clinically relevant tumour biomarkers (EGFR, EpCAM, CD24 and GPA33) of colorectal cancer (CRC), we classified plasma samples from 102 patients with CRC and 40 non-CRC controls with accuracies of more than 96%, prospectively assessed a cohort of 90 patients, for whom the burden of tumour EVs was predictive of five-year disease-free survival, and longitudinally analysed plasma from 11 patients, for whom the EV burden declined after surgery and increased on relapse. Rapid assays for the detection of combinations of tumour biomarkers in plasma EVs may aid cancer detection and patient monitoring.

原文???core.languages.en_GB???
頁(從 - 到)678-689
頁數12
期刊Nature Biomedical Engineering
5
發行號7
DOIs
出版狀態已出版 - 7月 2021

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