An electrochemical biosensor of the ketone 3-β-hydroxybutyrate for potential diabetic patient management

Lei Fang, Shih Han Wang, Chung Chiun Liu

研究成果: 雜誌貢獻期刊論文同行評審

28 引文 斯高帕斯(Scopus)

摘要

An electrochemical biosensor was developed for the monitoring of the concentration of ketone 3-β-hydroxybutyrate (3HB) in a physiological fluid for the potential diabetic patient management. Current electrochemical detection of 3HB involved at least two stepwise reactions, which may also require a mediator to facilitate the electron transfer. The detection method in this study involved only a single reaction step without any mediator. This biosensor operated at a relatively low electrochemical potential (+200 mV versus Ag/AgCl), and enzyme 3-hydroxybutyrate dehydrogenase (3HBDH, EC 1.1.1.30) was immobilized on thick film screen-printed iridium-modified working electrode detecting NADH (nicotinamide adenine dinucleotide, reduced form), which was the reaction product of 3HB and NAD+ (nicotinamide adenine dinucleotide, oxidized form) in the presence of 3HBDH. Electrochemical measurements showed that this biosensor responded well to 3HB in both phosphate buffer and 100% bovine serum. The reproducibility and the interference of this biosensor were studied and assessed. Spectrometric measurements of the ketone 3-β-hydroxybutyrate were carried out and were used to compare the electrochemical outputs of this biosensor, and the biosensor performed very well compared to the spectrometric study.

原文???core.languages.en_GB???
頁(從 - 到)818-825
頁數8
期刊Sensors and Actuators, B: Chemical
129
發行號2
DOIs
出版狀態已出版 - 22 2月 2008

指紋

深入研究「An electrochemical biosensor of the ketone 3-β-hydroxybutyrate for potential diabetic patient management」主題。共同形成了獨特的指紋。

引用此