An aptamer sandwich assay for protein biomarker detection by solution-gated silicon nanowire field-effect transistor systems

Cao An Vu, Wen Wang Yang, Hardy Wai Hong Chan, Wen Yih Chen

研究成果: 雜誌貢獻期刊論文同行評審

摘要

This study is the debut of a sandwich aptamer architecture for protein biomarker detection by field-effect transistor (FET) biosensors with cardiac Troponin I (cTnI) as a representative biomarker. Silicon nanowire FETs (SiNWFETs) were modified with mixed self-assembled monolayers of silane-PEG and glutaraldehyde to immobilize cTnI capturing aptamers (Tro4) and detect cTnI at different ultra-low concentrations. cTnI detection aptamers (Tro6) were then used to amplify the signal change from detecting cTnI by the fabricated SiNWFET aptasensors. The signal changes induced from cTnI detection and Tro6 amplification were recorded and calculated in different sensing buffers (10 mM BTP, 50 mM BTP, 150 mM BTP, and 1 × PBS) to determine the condition which can yield the maximal values for protein detection and signal amplification by the solution-gated SiNWFET biosensors. ELISA was used to investigate the effect of the aforementioned sensing buffers to the probe-target binding. Empirical results revealed that although diluted sensing buffers can produce stronger signal changes for protein detection by solution-gated SiNWFET biosensors compared to physiological conditions, probe-target binding is severely hampered. These results indicate that using diluted buffers for protein detection is not optimal, and there are other critical factors that need to be considered when designing operation conditions for protein sensing by FET biosensors. Based on the results obtained, we proposed a novel method and mathematical model using capacitance to interpret the electrical behavior of detecting protein biomarkers by solution-gated FET biosensors. The developed method and sandwich solution-gated SiNWFET aptasensors are applicable to quantify cTnI in different bio-samples.

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文章編號112245
期刊Microchemical Journal
207
DOIs
出版狀態已出版 - 12月 2024

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