An ABA-responsive bZIP protein, OsBZ8, mediates sugar repression of α-amylase gene expression

Expression of some α-amylase genes in cereals is suppressed by sugars and activated by sugar starvation. A 100-bp sugar response sequence (SRS) identified in the promoter of a rice α-amylase gene, αAmy3, contains three essential motifs: the GC box, the G box, and the TATCCA element. To study the mechanism of sugar regulation of αAmy3 transcription, an ABA-responsive bZIP protein, OsBZ8, which binds specifically to the G box in αAmy3 SRS was characterized and function analysed. In sucrose-starved rice suspension cells and embryos, decline in OsBZ8 mRNA levels coincided with the induction of αAmy3 mRNA accumulation. In vivo gain- and loss-of-function studies by transient expression assays in rice embryos revealed that OsBZ8 suppresses SRS activity through the G box and overrides the activity of an activator, OsMYBS1, which binds to the TATCCA element. Gel mobility shift assays revealed that OsBZ8 binds specifically to the G box in vitro. These studies suggest that OsBZ8 is a suppressor responsible for sugar repression of αAmy3 expression, and OsMYBS1 is responsible for sugar starvation induced expression of αAmy3.