A study on the binding ability of truncated aptamers for the prostate specific antigen using both computational and experimental approaches

Hui Ting Lin, Wei Yang, Wen Yu Su, Chun Ju Chan, Wen Yih Chen, Jeffrey J.P. Tsai, Wen Pin Hu

研究成果: 書貢獻/報告類型會議論文篇章同行評審

摘要

Prostate-specific antigen (PSA) test is a commonly used clinical examination to evaluate the risk of prostate cancer, with the antibodies used normally as the recognition molecules for measuring PSA levels in serum. Alternatively, aptamers that are able to bind target molecules with high affinity and specificity similar to antibodies could be generated much easier and cheaper than the production of antibodies. In this study, we used computaional and experimental approaches to select truncated PSA-binding aptamers generated from the sequence information of PSA-binding aptamers previously reported in a literature. Genetic algorithm, the analysis of secondary structure, and molecular simulation were utilized in the in silico analysis. The top 4 ranked sequecnes in silico analysis were evaluated through their PSA-binding ability on the quartz crystal microbalance (QCM) biosensor. Finally, We identified a truncated aptamer obtained from the selection showing a nearly 3.5-fold higher measured signal than the response produced by the best known DNA sequence in the QCM measurement.

原文???core.languages.en_GB???
主出版物標題Frontier Computing - Theory, Technologies and Applications FC 2017
編輯Neil Y. Yen, Jason C. Hung, Lin Hui
發行者Springer Verlag
頁面46-55
頁數10
ISBN(列印)9789811073977
DOIs
出版狀態已出版 - 2018
事件6th International Conference on Frontier Computing, FC 2017 - Osaka, Japan
持續時間: 12 7月 201714 7月 2017

出版系列

名字Lecture Notes in Electrical Engineering
464
ISSN(列印)1876-1100
ISSN(電子)1876-1119

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???event.eventtypes.event.conference???6th International Conference on Frontier Computing, FC 2017
國家/地區Japan
城市Osaka
期間12/07/1714/07/17

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