A single-use, disposable iridium-modified electrochemical biosensor for fructosyl valine for the glycoslated hemoglobin detection

Lei Fang, Wei Li, Yan Zhou, Chung Chiun Liu

研究成果: 雜誌貢獻期刊論文同行評審

50 引文 斯高帕斯(Scopus)

摘要

A single-use, disposable biosensor prototype of fructosyl valine (FV) was developed in this study. FV was a model compound for the glycosylated hemoglobin (HbA1c) detection and the measurement of HbA1c level was important for the long-term management of diabetic patients. Therefore, the detection of FV would be important for diabetic patient management as a means for HbA1c biosensing, particularly if this biosensor could be a single-use, disposable and manufactured cost effectively. This single-use, disposable FV electrochemical biosensor was fabricated using thick film screen-printing technique. Iridium nano-particle catalysts were incorporated in the carbon-based working and counter electrodes enhancing the performance of the biosensor for the FV detection. The enzyme fructosyl amine oxidase (FAO, EC 1.5.3, 0.45 U mg-1 of solid from recombinant Escherichia coli) was immobilized on the working electrode of the biosensor prototype. This biosensor operated at a relatively low electrochemical potential (+0.25 V versus Ag/AgCl) at ambient temperature to detect the enzymatically produced H2O2 from FV. This biosensor responded well to the FV level in a physiological range and the sensor output could be modeled by the Michaelis-Menton relationship. Thus, this biosensor prototype could be used effectively as the basis for a single-use, disposable HbA1c detection for diabetic patient management.

原文???core.languages.en_GB???
頁(從 - 到)235-238
頁數4
期刊Sensors and Actuators, B: Chemical
137
發行號1
DOIs
出版狀態已出版 - 28 3月 2009

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