A recombinant rice 16.9-kda heat shock protein can provide thermoprotection in vitro

Ching Hui Yeh, Kai Wun Yeh, Shu Hsing Wu, Pi Fang Linda Chang, Yih Ming Chen, Chu Yung Lin

研究成果: 雜誌貢獻期刊論文同行評審

28 引文 斯高帕斯(Scopus)

摘要

It is difficult to obtain large amounts of purified low-molecular-mass heat shock proteins (LMM HSPs), which are unique to plants, for biochemical and physiological studies. Therefore, an attempt was made to produce such a HSP by applying recombinant DNA technology. We fused the cDNA for a rice class I 16.9-kDa HSP, pTSl, to the gene for glutathione S-transferase (GST) of Schistosoma japonicum and we obtained large amounts of the fusion protein from transformed Escherichia coli cells. In addition, we found that the 16.9-kDa HSP obtained by cleavage of the recombinant protein could also form a protein complex of ̃310 kDa under non-denaturing conditions as can the small, native, class I HSPs from heat-shocked rice seedlings. An assay in vitro to examine the thermoprotection of rice soluble proteins from heat denaturation revealed the strong stabilizing effect of the recombinant HSP.

原文???core.languages.en_GB???
頁(從 - 到)1341-1348
頁數8
期刊Plant and Cell Physiology
36
發行號7
出版狀態已出版 - 10月 1995

指紋

深入研究「A recombinant rice 16.9-kda heat shock protein can provide thermoprotection in vitro」主題。共同形成了獨特的指紋。

引用此