A novel application of indolicidin for gene delivery

Wei Wen Hu; Ze Wei Lin; Ruoh Chyu Ruaan; Wen Yih Chen; Shiow Lian Catherine Jin; Yung Chang

doi:10.1016/j.ijpharm.2013.08.035

A novel application of indolicidin for gene delivery

Wei Wen Hu, Ze Wei Lin, Ruoh Chyu Ruaan, Wen Yih Chen, Shiow Lian Catherine Jin, Yung Chang

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

14 引文斯高帕斯（Scopus）

摘要

A bovine derived antimicrobial peptide, indolicidin (IL), was studied of its new application for gene transfer. Plasmid DNA was complexed with both IL and polyethylenimine (PEI) as ternary particles. Compared to DNA/IL complexes, the DNA/IL/PEI particles demonstrated high zeta potentials, small particle sizes, and superior loading efficiencies, suggesting the incorporation of polycations can support IL for gene delivery. For in vitro experiments, these ternary particles significantly improved gene transfection efficiencies over the sole administrations of IL or PEI. This synergistic effect revealed that IL and PEI may play different roles for gene transfer. Our results suggest that IL should be a potential carrier for gene delivery. As our knowledge, our study should be the first article indicating the carrier ability of IL for gene transfer.

原文	???core.languages.en_GB???
頁（從 - 到）	293-300
頁數	8
期刊	International Journal of Pharmaceutics
卷	456
發行號	2
DOIs	https://doi.org/10.1016/j.ijpharm.2013.08.035
出版狀態	已出版 - 2013

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10.1016/j.ijpharm.2013.08.035

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title = "A novel application of indolicidin for gene delivery",

abstract = "A bovine derived antimicrobial peptide, indolicidin (IL), was studied of its new application for gene transfer. Plasmid DNA was complexed with both IL and polyethylenimine (PEI) as ternary particles. Compared to DNA/IL complexes, the DNA/IL/PEI particles demonstrated high zeta potentials, small particle sizes, and superior loading efficiencies, suggesting the incorporation of polycations can support IL for gene delivery. For in vitro experiments, these ternary particles significantly improved gene transfection efficiencies over the sole administrations of IL or PEI. This synergistic effect revealed that IL and PEI may play different roles for gene transfer. Our results suggest that IL should be a potential carrier for gene delivery. As our knowledge, our study should be the first article indicating the carrier ability of IL for gene transfer.",

keywords = "Cell-penetrating peptide, Gene delivery, Indolicidin, Nonviral vectors, Transfection",

author = "Hu, {Wei Wen} and Lin, {Ze Wei} and Ruaan, {Ruoh Chyu} and Chen, {Wen Yih} and Jin, {Shiow Lian Catherine} and Yung Chang",

note = "Funding Information: This research was supported by the grant of NSC 98-2218-E-008-009 from the National Science Council of Taiwan . ",

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T1 - A novel application of indolicidin for gene delivery

AU - Hu, Wei Wen

AU - Lin, Ze Wei

AU - Ruaan, Ruoh Chyu

AU - Chen, Wen Yih

AU - Jin, Shiow Lian Catherine

AU - Chang, Yung

N1 - Funding Information: This research was supported by the grant of NSC 98-2218-E-008-009 from the National Science Council of Taiwan .

PY - 2013

Y1 - 2013

N2 - A bovine derived antimicrobial peptide, indolicidin (IL), was studied of its new application for gene transfer. Plasmid DNA was complexed with both IL and polyethylenimine (PEI) as ternary particles. Compared to DNA/IL complexes, the DNA/IL/PEI particles demonstrated high zeta potentials, small particle sizes, and superior loading efficiencies, suggesting the incorporation of polycations can support IL for gene delivery. For in vitro experiments, these ternary particles significantly improved gene transfection efficiencies over the sole administrations of IL or PEI. This synergistic effect revealed that IL and PEI may play different roles for gene transfer. Our results suggest that IL should be a potential carrier for gene delivery. As our knowledge, our study should be the first article indicating the carrier ability of IL for gene transfer.

AB - A bovine derived antimicrobial peptide, indolicidin (IL), was studied of its new application for gene transfer. Plasmid DNA was complexed with both IL and polyethylenimine (PEI) as ternary particles. Compared to DNA/IL complexes, the DNA/IL/PEI particles demonstrated high zeta potentials, small particle sizes, and superior loading efficiencies, suggesting the incorporation of polycations can support IL for gene delivery. For in vitro experiments, these ternary particles significantly improved gene transfection efficiencies over the sole administrations of IL or PEI. This synergistic effect revealed that IL and PEI may play different roles for gene transfer. Our results suggest that IL should be a potential carrier for gene delivery. As our knowledge, our study should be the first article indicating the carrier ability of IL for gene transfer.

KW - Cell-penetrating peptide

KW - Gene delivery

KW - Indolicidin

KW - Nonviral vectors

KW - Transfection

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SN - 0378-5173

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SP - 293

EP - 300

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

IS - 2

ER -

A novel application of indolicidin for gene delivery

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