This study is to construct a non-contact pulse automatic positioning measurement system for Traditional Chinese Medicine (TCM) using optical triangulation measurements. The system consists of a linear laser, a CMOS image sensor and image analysis software. The linear laser is projected on the pulse beat location on the wrists; the CMOS image sensor records the process and the software analyzes the images. The program mainly uses the optical centroid and fast Fourier transform (FFT) principles to calculate centroid changes (pulse amplitude changes) from the images taken by the CMOS image sensor. It returns the positions of cun, guan and chi pulses automatically in terms of the amplitudes and the signals are then transformed from the time domain (time-amplitude) into the frequency domain (frequency-amplitude) via FFT to obtain the waveforms and frequencies of the cun, guan and chi pulses. It successfully extracts the data from the TCM pulse reading and can be a medical aid system for TCM. Combining the advantages of optical measurement and computer automation, this system provides a non-contact, easy to operate, fast in detection and low-cost equipment design.