@article{1dba8a54743f4fd2850d3d1b3034f6de,
title = "A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues",
abstract = "Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 μm, and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes (Oct4, Sox2, Klf4 and Nanog) compared with cells isolated using the conventional culture method.",
author = "Akon Higuchi and Wang, {Ching Tang} and Ling, {Qing Dong} and Lee, {Henry Hsin Chung} and Kumar, {S. Suresh} and Yung Chang and Alarfaj, {Abdullah A.} and Munusamy, {Murugan A.} and Hsu, {Shih Tien} and Wu, {Gwo Jang} and Akihiko Umezawa",
note = "Funding Information: This research was partially supported by the Ministry of Science and Technology, Taiwan, under the grant numbers 103-2120-M-008-001 and 102-2221-E-008-112-MY2. This work was also supported by the Landseed Hospital project (NCU-LSH-102-A-003 and 103LSH-NCU-1), the National Defense Medical Center Project (102NCU-NDMC-01), and the Cathay General Hospital Project (102NCU-CGH-02, 103CGH-NCU-A3 and CGH-MR-A10204 and CGH-MR-A10301). A Grant-in-Aid for Scientific Research (number 24560968) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan is also acknowledged. We acknowledge the International High Cited Research Group (IHCRG #14-104), Deanship of Scientific Research, King Saud University, Riyadh, Kingdom of Saudi Arabia.",
year = "2015",
month = may,
day = "13",
doi = "10.1038/srep10217",
language = "???core.languages.en_GB???",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
}