Neem cake as a promising larvicide and adulticide against the rural malaria vector Anopheles culicifacies (Diptera: Culicidae): a HPTLC fingerprinting approach

  • Kalimuthu Kovendan (貢獻者)
  • Balamurugan Chandramohan (貢獻者)
  • Giovanni Benelli (貢獻者)
  • Higuchi Akon (貢獻者)
  • Marimuthu Govindarajan (貢獻者)
  • Angelo Canale (貢獻者)
  • Devakumar Dinesh (貢獻者)
  • Kadarkarai Murugan (貢獻者)
  • Marcello Nicoletti (貢獻者)
  • Pari Madhiyazhagan (貢獻者)
  • Chellasamy Panneerselvam (貢獻者)
  • Chiara Toniolo (貢獻者)



Mosquitoes are insects of huge public health importance, since they act as vectors for important pathogens and parasites. Here, we focused on the possibility of using the neem cake in the fight against mosquito vectors. The neem cake chemical composition significantly changes among producers, as evidenced by our HPTLC (High performance thin layer chromatography) analyses of different marketed products. Neem cake extracts were tested to evaluate the ovicidal, larvicidal and adulticidal activity against the rural malaria vector Anopheles culicifacies. Ovicidal activity of both types of extracts was statistically significant, and 150 ppm completely inhibited egg hatching. LC50 values were extremely low against fourth instar larvae, ranging from 1.321 (NM1) to 1.818 ppm (NA2). Adulticidal activity was also high, with LC50 ranging from 3.015 (NM1) to 3.637 ppm (NM2). This study pointed out the utility of neem cake as a source of eco-friendly mosquitocides in Anopheline vector control programmes.
可用日期1 1月 2017
發行者figshare Academic Research System