Xeno-free culture and proliferation of hPSCs on 2D biomaterials

Ting Wang, Tao Yu, Chang Yen Tsai, Zhao Yu Hong, Wen Hui Chao, Yi Shuo Su, Suresh Kumar Subbiah, Remya Rajan Renuka, Shih Tien Hsu, Gwo Jang Wu, Akon Higuchi

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Human pluripotent stem cells (human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs)) have unlimited proliferative potential, whereas adult stem cells such as bone marrow-derived stem cells and adipose-derived stem cells have problems with aging. When hPSCs are intended to be cultured on feeder-free or xeno-free conditions without utilizing mouse embryonic fibroblasts or human fibroblasts, they cannot be cultured on conventional tissue culture polystyrene dishes, as adult stem cells can be cultured but should be cultivated on material surfaces grafted or coated with (a) natural or recombinant extracellular matrix (ECM) proteins, (b) ECM protein-derived peptides and specific synthetic polymer surfaces in xeno-free and/or chemically defined conditions. This review describes current developing cell culture biomaterials for the proliferation of hPSCs while maintaining the pluripotency and differentiation potential of the cells into 3 germ layers. Biomaterials for the cultivation of hPSCs without utilizing a feeder layer are essential to decrease the risk of xenogenic molecules, which contributes to the potential clinical usage of hPSCs. ECM proteins such as human recombinant vitronectin, laminin-511 and laminin-521 have been utilized instead of Matrigel for the feeder-free cultivation of hPSCs. The following biomaterials are also discussed for hPSC cultivation: (a) decellularized ECM, (b) peptide-grafted biomaterials derived from ECM proteins, (c) recombinant E-cadherin-coated surface, (d) polysaccharide-immobilized surface, (e) synthetic polymer surfaces with and without bioactive sites, (f) thermoresponsive polymer surfaces with and without bioactive sites, and (g) synthetic microfibrous scaffolds.

Original languageEnglish
Title of host publicationProgress in Molecular Biology and Translational Science
EditorsAkon Higuchi, Yinghong Zhou, Shih-Hwa Chiou
PublisherElsevier B.V.
Number of pages45
ISBN (Print)9780443134111
StatePublished - Jan 2023

Publication series

NameProgress in Molecular Biology and Translational Science
ISSN (Print)1877-1173
ISSN (Electronic)1878-0814


  • Biomaterial
  • Cell cultivation
  • Cell differentiation
  • Embryonic stem cells
  • Extracellular matrix
  • Hydrogel
  • Induced pluripotent stem cells
  • Peptide


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